Thymineless Mutagenesis in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Thymineless death in Escherichia coli.
There is little doubt that thymine starvation af fects the DNA of thymine requiring bacteria1-5. However, the process of killing is fairly complicated and remains to be clarified. The genes responsible for the control of sensitivity to thymine starvation have not been identified, even though claims have been but forward for at least one gene6. Besides, whether the cells die when still in incub...
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Thymine deprivation can be achieved in bacteriophage T4 either by the use of the thymidylate synthetase inhibitor FUdR, or by an appropriate combination of genetic blocks; both methods produce marked mutagenesis. Extensive tests of the specificity of thymineless mutagenesis reveal that only A:T base pairs are affected, and that transitions and possibly transversions are produced. This system th...
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Freifelder, David (University of California, Berkeley), and Ole Maaløe. Energy requirement for thymineless death in cells of Escherichia coli. J. Bacteriol. 88:987-990. 1964.-Thymineless death in thymine-requiring Escherichia coli is arrested immediately and reversibly by nitrogenation if the bacterial population is growing in a medium containing a carbon source that can only be metabolized aer...
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Thymineless death was examined in Escherichia coli 15T(-) and recombinants of 15T(-) and E. coli K-12. Those strains that were very sensitive to thymine deprivation were also very sensitive to a variety of inducing agents (mitomycin C, ultraviolet light, hydroxyurea, and nalidixic acid). Those strains that were relatively resistant to thymineless death were also relatively resistant to the indu...
متن کاملThymineless death in polA+ and polA- strains of Escherichia coli.
The extreme sensitivity of polA(-) cells to thymineless death is due, primarily, to the absence of an extended lag prior to the commencement of death. Once thymineless death has commenced, the rate in polA(-) cells is only slightly faster than in polA(+) cells.
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1974
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.119.3.861-867.1974